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filamented borosilicate glass pipettes  (Sutter Instrument Company)


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    Structured Review

    Sutter Instrument Company filamented borosilicate glass pipettes
    Filamented Borosilicate Glass Pipettes, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 98/100, based on 3829 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/filamented+borosilicate+glass+pipettes/Borosilicate+Glass/pm41981306-310-5-9
    Average 98 stars, based on 3829 article reviews
    filamented borosilicate glass pipettes - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    other:

    Article Title: Mapping Kenyon cell inputs in Drosophila using dye electroporation
    Article Snippet: Note: The parameters that we use to pull electrodes are optimized for the P-2000 laser-based micropipette puller system and filamented borosilicate glass pipettes (Sutter Instrument).

    Article Title: Disease-causing Slack potassium channel mutations produce opposite effects on excitability of excitatory and inhibitory neurons.
    Article Snippet: To determine the distance from the soma to the AIS start point, the shortest possible distance between the neuronal soma (identified by DAPI or VGlut1/GAD67 staining) and themost proximal portion of the AIS (identified by AnkG/NaV1.6 staining) was measured using the straight-line tool in Leica LAS-X.34,59,60 For the assessment of AIS morphology in primary cortical cultures, nine embryos or cultures were used for each genotype.

    Immunohistochemistry:

    Article Title: Interaction Between HCN and Slack Channels Regulates mPFC Pyramidal Cell Excitability in Working Memory Circuits.
    Article Snippet: .. Recording electrodes were pulled from filamented borosilicate glass pipettes (Sutter Instrument, CA), and had tip resistances between 4 and 6 MΩ when filled with the following internal solution (in mM): 124 K-gluconate, 2 A gold (UMC, Utrecht, The Netherlands) for 30 min. For gold-gold dual-labeling immunohistochemistry, grids were incubated with anti-HCN1 and anti-Slack primary antibodies 1:100 overnight, rinsed in TBS then incubated with 15 nm (HCN1) and 5 nm (Slack) protein A gold (UMC, Utrecht, The Netherlands) for 30 min. ..

    Incubation:

    Article Title: Interaction Between HCN and Slack Channels Regulates mPFC Pyramidal Cell Excitability in Working Memory Circuits.
    Article Snippet: .. Recording electrodes were pulled from filamented borosilicate glass pipettes (Sutter Instrument, CA), and had tip resistances between 4 and 6 MΩ when filled with the following internal solution (in mM): 124 K-gluconate, 2 A gold (UMC, Utrecht, The Netherlands) for 30 min. For gold-gold dual-labeling immunohistochemistry, grids were incubated with anti-HCN1 and anti-Slack primary antibodies 1:100 overnight, rinsed in TBS then incubated with 15 nm (HCN1) and 5 nm (Slack) protein A gold (UMC, Utrecht, The Netherlands) for 30 min. ..

    Saline:

    Article Title: Mapping Kenyon cell inputs in Drosophila using dye electroporation.
    Article Snippet: Saline solution Reagent Final concentration Amount NaCl 108 mM 12.62 g KCl 5 mM 0.75 g HEPES 5 mM 2.38 g Trehalose 5 mM 3.78 g Sucrose 10 mM 6.85 g NaHCO3 4 mM 0.67 g NaH2PO4 1 mM 0.24 g MgCl2 4 mM 8 mL CaCl2 2 mM 4 mL ddH2O N/A Up to 2 L Parameters for pulling electrodes Parameter Value Delay 200 Filament 4 Heat 340 Number of lines (cycles) 1 Number of loops 4 Time of last loop 5.4–5.85 s Note: The parameters that we use to pull electrodes are optimized for the P-2000 laser-based micropipette puller system and filamented borosilicate glass pipettes (Sutter Instrument). .. Saline solution Reagent Final concentration Amount NaCl 108 mM 12.62 g KCl 5 mM 0.75 g HEPES 5 mM 2.38 g Trehalose 5 mM 3.78 g Sucrose 10 mM 6.85 g NaHCO3 4 mM 0.67 g NaH2PO4 1 mM 0.24 g MgCl2 4 mM 8 mL CaCl2 2 mM 4 mL ddH2O N/A Up to 2 L Parameters for pulling electrodes Parameter Value Delay 200 Filament 4 Heat 340 Number of lines (cycles) 1 Number of loops 4 Time of last loop 5.4–5.85 s Note: The parameters that we use to pull electrodes are optimized for the P-2000 laser-based micropipette puller system and filamented borosilicate glass pipettes (Sutter Instrument). ..

    Concentration Assay:

    Article Title: Mapping Kenyon cell inputs in Drosophila using dye electroporation.
    Article Snippet: Saline solution Reagent Final concentration Amount NaCl 108 mM 12.62 g KCl 5 mM 0.75 g HEPES 5 mM 2.38 g Trehalose 5 mM 3.78 g Sucrose 10 mM 6.85 g NaHCO3 4 mM 0.67 g NaH2PO4 1 mM 0.24 g MgCl2 4 mM 8 mL CaCl2 2 mM 4 mL ddH2O N/A Up to 2 L Parameters for pulling electrodes Parameter Value Delay 200 Filament 4 Heat 340 Number of lines (cycles) 1 Number of loops 4 Time of last loop 5.4–5.85 s Note: The parameters that we use to pull electrodes are optimized for the P-2000 laser-based micropipette puller system and filamented borosilicate glass pipettes (Sutter Instrument). .. Saline solution Reagent Final concentration Amount NaCl 108 mM 12.62 g KCl 5 mM 0.75 g HEPES 5 mM 2.38 g Trehalose 5 mM 3.78 g Sucrose 10 mM 6.85 g NaHCO3 4 mM 0.67 g NaH2PO4 1 mM 0.24 g MgCl2 4 mM 8 mL CaCl2 2 mM 4 mL ddH2O N/A Up to 2 L Parameters for pulling electrodes Parameter Value Delay 200 Filament 4 Heat 340 Number of lines (cycles) 1 Number of loops 4 Time of last loop 5.4–5.85 s Note: The parameters that we use to pull electrodes are optimized for the P-2000 laser-based micropipette puller system and filamented borosilicate glass pipettes (Sutter Instrument). ..



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